In vitro safety assessment of reduced graphene oxide in human monocytes and T cells.

• DOI: 10.1016/j.envres.2023.116356
• Publication Date: 2023-06-07T23:13:46Z
• Authors: Óscar Cebadero-Do...; Antonio Casas-Rod...; María Puerto; Ana María Cameán; Angeles Jos
• Keywords: Jurkat cells; THP1 cell line; Monocyte

Considering the increase in use of graphene derivatives different fields, environmental and human exposure to these materials is likely, potential consequences are not fully elucidated. This study focused on immune system, as this plays a key role organism's homeostasis. In sense, cytotoxicity response reduced oxide (rGO) was investigated monocytes (THP-1) T cells (Jurkat). A mean effective concentration (EC50-24 h) 121.45 ± 11.39 μg/mL 207.51 21.67 for obtained THP-1 Jurkat cells, respectively. rGO decreased differentiation at highest after 48 h exposure. Regarding inflammatory genetic level, upregulated IL-6 all cytokines tested 4 At 24 h, upregulation maintained, significant decrease TNF-α gene expression observed cells. Moreover, TNF-α, INF-γ were maintained With respect apoptosis/necrosis, altered but down regulation BAX BCL-2 These genes showed values closer negative control h. Finally, did trigger release any cytokine time assayed. conclusion, our data contributes risk assessment material suggest that has an impact system whose final should be further investigated.