Transgenerational effects of the levonorgestrel-based birth control pill in zebrafish offspring

• DOI: 10.1016/j.etap.2024.104540
• Publication Date: 2024-08-23T00:53:15Z
• Authors: Milena Fortuna; Amanda Carolina C...; Lisiane Siqueira; Suelen Mendonça S...; Natália Freddo; Jéssica Nardi; Ísis Piasson Barl...; Milena Zanoello B...; Leonardo José Gil...
• Keywords: Male; Embryo, Nonmammalian; Larva; Animals; Female; Levonorgestrel; Zebrafish; Water Pollutants, Chemical

The consumption of hormone-derived medicines, such as levonorgestrel (LNG), is increasing worldwide, and its discharge into the environment reaches non-target organisms. In our previous study, we exposed the parental generation of zebrafish to environmentally relevant concentrations of LNG during the developmental phase. Subsequently, they had grown in a tank with clean water until adulthood. Now, we allowed this parental generation to reproduce to obtain F1 progeny unexposed to LGN, in order to analyze the transgenerational effects of parental LNG exposure on the survival and hatching of unexposed F1 embryos and the stress and behavior of F1 larvae. Here, we found decreased survival rates with higher LNG concentrations, providing a transgenerational effect. This highlights the environmental impact of exposure to LNG, causing damage at the individual and population level and affecting the next generation at the beginning of development, impacting qualities in the survival of the species.