Optimized strategy valorizing unautoclaved cottonseed hull as ruminant alternative feeds via solid-state fermentation: Detoxifying polyphenols, restraining hazardous microflora and antibiotic-resistance gene hosts

• DOI: 10.1016/j.eti.2022.102937
• Publication Date: 2022-10-27T23:59:05Z
• Authors: Guilin Du; Yan Zhou; Jingxian Zhang; Suwan Han; Xiangcen Liu; Chenyang Yuan; Fabrice Ndayisenga; Jiping Shi; Baoguo Zhang
• Keywords: Solid-State Fermentation

Cottonseed hull (CSH), typical agro-industrial byproduct, is widely valorized as ruminant alternative feeds, but many constraints including extensive anti-nutritional factors and indigenous hazardous microflora are still reasonably unsolved. This study proposed a novel strategy that valorizes unautoclaved CSH feeds via solid-state fermentation (SSF), where feasibility microbial mechanism were roundly investigated. The SSF of raw (R group, in this study) was compared with autoclaved (M prevalent strategy) based on physicochemical properties, chemical functionalities, superficial structures. Compared to M R group though has exhibited near detoxification effect polyphenols (81.7% free gossypol (FG) 62.2% condensed tannin (CT), both below threshold values) after SSF, yet the accumulation crude protein fat been higher (109.3 51.0 g/kg dry matter, respectively). Metagenomic analyses indicated microorganisms antibiotic-resistance genes prevented from amplification group. More importantly, Pleurotus keystone contributor functional gene sets degrading (FG CT) lignin during group; furthermore, CAZyme associated polysaccharide degradation primarily derived bacterial communities (Cellulomonas, Stenotrophomonas, et al.). Ultimately, can be feasible minimum facility investment energy consumption, thus expediting valorization feedstock industries sustainable bio-economy developing countries.