Substance P, NPY, CCK and their receptors in five brain regions in major depressive disorder with transcriptomic analysis of locus coeruleus neurons

• DOI: 10.1016/j.euroneuro.2023.09.004
• Publication Date: 2023-11-04T18:02:38Z
• Authors: Swapnali Barde; Julio Aguila; Wen Zhong; Anna Solarz; Irene Mei; Josee Prud'homme; Miklos Palkovits; Gustavo Turecki; Jan Mulder; Mathias Uhlén; Corina Nagy; Naguib Mechawar; Eva Hedlund; Tomas Hökfelt
• Keywords: Locus coeruleus; Dorsal raphe nucleus

Major depressive disorder (MDD) is a serious disease and burden to patients, families society. Rodent experiments human studies suggest that several neuropeptide systems are involved in mood regulation. The aim of this study two-fold: (i) monitor, with qPCR, transcript levels the substance P/tachykinin (TAC), NPY CCK bulk samples from control suicide subjects, targeting five postmortem brain regions including locus coeruleus (LC); (ii) analyse expression family transcripts LC neurons 'normal' brains by using laser capture microdissection Smart-Seq2 RNA sequencing. qPCR revealed distinct regional patterns male female controls higher for TAC system dorsal raphe nucleus LC, versus prefrontal cortex. In TAC, receptors few were increased mainly cortex LC. second on noradrenergic GAL, NPY, TAC1, CCK, TACR1 many other peptides (e.g. Cerebellin4 CARTPT) Adcyap1R1 GPR173). These data our previous results indicates tachykinin galanin may be valid targets developing antidepressant medicines. Moreover, perturbation MDD detection further receptor shed new light signalling mechanisms possibly associated disorders.