Anthelmintic efficacy of an organic fraction from Guazuma ulmifolia leaves and evaluation of reactive oxidative stress on Haemonchus contortus

• DOI: 10.1016/j.exppara.2024.108768
• Publication Date: 2024-04-27T00:44:02Z
• Authors: Guillermo Reséndi...; Agustín Olmedo-Ju...; Roberto González-...; Jorge Alberto Cor...; Manasés González-...; Ana Elvia Sánchez...; María Eugenia Lóp...; Crisóforo Mercado...; Alejandro Lara-Bueno; Rosa Isabel Higue...

This study describes the anthelmintic efficacy of an organic fraction (EtOAc-F) from Guazuma ulmifolia leaves and evaluation its reactive oxidative stress on Haemonchus contortus. The first step was to assess effect EtOAc-F at 0.0, 3.5, 7.0 14 mg kg body weight (BW) in gerbil's (Meriones unguiculatus) artificially infected with H. contortus infective larvae (L3). second evaluate preliminary toxicity after oral administration gerbils. Finally, third determine relative expression biomarkers such as glutathione (GPx), catalase (CAT), superoxide dismutase (SOD) against L3 post-exposition EtOAc-F. Additionally, less-polar compounds were identified by gas mass spectrophotometry (GC-MS). highest (97.34%) found gerbils treated mg/kg BW. Histopathological analysis did not reveal changes tissues. reflects overexpression GPx (p<0.05, fold change: 14.35) over SOD (p≤0.05, 0.18) exposed 97.44 mg/mL compared negative control. GC-MS revealed presence 4-hydroxybenzaldehyde (1), leucoanthocyanidin derivative (2), coniferyl alcohol (3), ferulic acid methyl ester acetate (4), 2,3,4-trimethoxycinnamic (5) epiyangambin (6) major compounds. According these results, G. exhibit increased