Antioxidant interactions between hydrophilic and lipophilic phytochemicals are influenced by their ratios and total concentrations

• DOI: 10.1016/j.fbio.2021.101465
• Publication Date: 2021-11-21T20:46:15Z
• Authors: Yao Pan; Hongyan Li; Xuan Chen; Yawei Fan; Bing Zhang; Rong Liu; Ze-yuan Deng
• Keywords: Carotene; p-Coumaric acid

Abstract Lipophilic (lycopene, β-carotene) and hydrophilic (caffeic acid, p-coumaric acid) phytochemicals were mixed in molar mass at different concentrations/ratios to explore the antioxidant interactions on H2O2-treated H9c2 cells. Reactive oxide species (ROS), expression of antioxidant enzymes, and oxidation products were analyzed and evaluated by synergistic rate (SR) and combination index (CI). At constant concentrations, antioxidant interactions were influenced by ratios. Group contained more hydrophilic compounds (eg., p-coumaric acid:β-carotene=9:1) showed antioxidant synergism (SR=22.33±1.23, SR>0) at 3.0 μM, while groups that contained more lipophilic compounds (eg., p-coumaric acid:β-carotene=1:9) showed antioxidant antagonism (SR=-11.68±1.25, SR 0) to antagonism (SR=-11.68±1.25, SR