A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium

• DOI: 10.1016/j.febslet.2004.09.084
• Publication Date: 2004-10-21T18:48:22Z
• Authors: Markus Fischer; Ilka Haase; Evelyn Simmeth; Günther Gerisch; Annette Müller-Ta...
• Keywords: 0301 basic medicine; Base Sequence; Actin cytoskeleton; Molecular Sequence Data; Microtubule; Dictyostelium discoideum; Fluorescent protein; Endocytosis; Luminescent Proteins; 03 medical and health sciences; Synthetic gene; Animals; Dictyostelium; Electrophoresis, Polyacrylamide Gel; Amino Acid Sequence; Cytoskeleton; In Situ Hybridization, Fluorescence; Monomeric RFP; Red Fluorescent Protein

Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of in live cells. However, the application these is restrained by stable oligomer formation and their weak fluorescence vivo. Previous attempts to eliminate problems mutagenesis RFP from Discosoma (DsRed) resulted monomeric mRFP1 tetrameric RedStar RFP, which distinguished its enhanced Based on mutations, we have generated an mRFPmars, report spectral properties. Together green labels, used mRFPmars visualize filamentous actin structures microtubules Dictyostelium This proved be suitable monitor dynamics cytoskeletal cell motility, mitosis, endocytosis using dual-wavelength microscopy.