DNA methylation modulatesSalmonella entericaserovar Typhimurium virulence inCaenorhabditis elegans

• DOI: 10.1016/j.femsle.2005.02.030
• Publication Date: 2005-03-08T23:31:07Z
• Authors: Javin P. Oza; Jimmy B. Yeh; Norbert O. Reich
• Keywords: Salmonella enterica

Salmonella enterica serovar Typhimurium was previously shown to be virulent in Caenorhabditis elegans. Here we demonstrate that DNA adenine methyltransferase (DAM) modulates virulence the nematode, as it does mice. After 5 days of continual exposure bacteria, twice many worms died when exposed wild-type than dam-mutant strain Salmonella. Similar trends were observed strains for h and transferred avirulent Escherichia coli OP50. While a 10-fold attenuation absence DAM, dam-strain still able infect persist host worm. Our results further support use C. elegans an accessible readily studied animal model bacterial pathogenesis. However, our suggest crucial responses differ between murine nematode models. Additionally, carried out preliminary liquid culture based experiments with long term goal developing high throughput screens DAM inhibitors.