Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method

• DOI: 10.1016/j.fshw.2021.02.019
• Publication Date: 2021-04-19T10:55:27Z
• Authors: Changqin Li; Yiping Cui; Jie Lu; Lijun Meng; Changyang Ma; Zhenhua Liu; Yan Zhang; Wenyi Kang
• Keywords: 0301 basic medicine; 03 medical and health sciences; Component knock-out; Nutrition. Foods and food supply; TX341-641; Ganoderma lucidum; BP neural network; RAW 264.7 cell; Spectrum-effect relationships; 3. Good health

This study was designed to elucidate the immunoregulation of Ganoderma lucidum. HPLC fingerprint and spectrum-effect relationship of G. lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy. Then the target compounds were identified by high resolution mass spectrometry. The results indicated that there are both enhanced immunity and immunosuppressive components in G. lucidum. BP neural network was trained with the common peak area and immune efficacy index of G. lucidum fingerprint as samples, and a combined evaluation system of G. lucidum fingerprint efficacy was established. The correlation coefficient R of BP network model was 0.986 43, and the error of pharmacodynamic prediction results was in the ideal range. Eight compounds were identified by high resolution mass spectrometry. The compounds related to immune activity in G. lucidum were determined in this study.