Antagonizing exosomal miR-18a-5p derived from prostate cancer cells ameliorates metastasis-induced osteoblastic lesions by targeting Hist1h2bc and activating Wnt/β-catenin pathway

• DOI: 10.1016/j.gendis.2022.06.007
• Publication Date: 2022-07-31T01:17:41Z
• Authors: Fanchun Zeng; Chunrong Zhao; Rujie Wang; Lingyan Ren; Hao Qiu; Zhi Zou; Haibin Ding; Zhongyi Sun; Jianmei Li; Shiwu Dong
• Keywords: Exosome

More than 50% of prostate cancer (PCa) patients have bone metastasis with osteoblastic lesions. MiR-18a-5p is associated the development and PCa, but it remains unclear whether involved in We first found that miR-18a-5p was highly expressed microenvironment PCa metastases. To address how affects lesions, antagonizing cells or pre-osteoblasts inhibited osteoblast differentiation vitro. Moreover, injection inhibition improved biomechanical properties mineral mass vivo. Furthermore, transferred to osteoblasts by exosomes derived from targeted Hist1h2bc gene, resulting Ctnnb1 up-regulation Wnt/β-catenin signaling pathway. Translationally, antagomir-18a-5p significantly alleviated sclerotic lesions metastases BALB/c nude mice. These data suggest exosome-delivered ameliorates PCa-induced