Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β

• DOI: 10.1016/j.heliyon.2020.e03493
• Publication Date: 2020-03-03T07:18:01Z
• Authors: Hiromasa Aoki; Misaki Yamashita; Tadahiro Hashita; Koichi Ogami; Shinichi Hoshino; Takahiro Iwao; Tamihide Matsunaga
• Keywords: Endothelial progenitor cell

Endothelial cells (ECs) and endothelial progenitor (EPCs) play crucial roles in maintaining vascular health homeostasis. Both cell types have been used regenerative therapy as well various vitro models; however, the properties of primary human ECs EPCs are dissimilar owing to differences genetic backgrounds sampling techniques. Human induced pluripotent stem (hiPSCs) an alternative source EPCs. However, low purity differentiated from hiPSCs, purification via antigen-antibody reaction, which damages cells, is indispensable. Besides, limited expandability, it difficult produce these large numbers. Here we report development relatively simple differentiation methods for hiPSC-derived (iEPCs). Furthermore, discovered that a combination three small molecules, is, Y-27632 (a selective inhibitor Rho-associated, coiled-coil containing protein kinase [ROCK]), A 83-01 receptor-like transforming growth factor beta [TGF-β]), CHIR-99021 glycogen synthase kinase-3β [GSK3β] also activates Wnt), dramatically stimulated synthesis-related pathways enhanced proliferative capacity iEPCs. These findings will help establish supply system at industrial scale.