A comparison of exosome purification methods using serum of Marek's disease virus (MDV)-vaccinated and -tumor-bearing chickens

• DOI: 10.1016/j.heliyon.2020.e05669
• Publication Date: 2020-12-12T01:19:40Z
• Authors: Sabari Nath Neeru...; Nicholas A. Egan; Joseph Patria; Imane Assakhi; Phaedra Tavlaride...; Shannon Modla; Eric R. Muñoz; Matthew B. Hudson; Mark S. Parcells
• Keywords: Marek's disease; Exosome

Marek's disease (MD) is an alphaherpesvirus (Marek's virus, MDV)-induced pathology of chickens associated with paralysis, immunosuppression, neurological signs, and T-cell lymphomas. MD controlled in poultry production via live attenuated vaccines. The purpose the current study was to compare methods for precipitating exosomes from vaccinated protected chicken sera (VEX) tumor-bearing (TEX) biomarker analysis vaccine-induced protection lymphomas respectively. A standard polyethylene glycol (PEG, 8%) method compared a commercial reagent (total exosome isolation reagent, TEI) yield RNA content. Although purified by PEG or TEI were comparable size morphology, TEI-reagent yielded 3-4-fold greater concentration. Relative expression 8 out 10 G. gallus- MDV1-encoded miRNAs examined displayed significant difference depending upon precipitation used. Standard yields comparable, albeit lower amounts than distinctive miRNA composition.