Development of ELISA against milk haptoglobin for diagnosis of subclinical mastitis in goats

• DOI: 10.1016/j.heliyon.2021.e06314
• Publication Date: 2021-02-23T02:53:39Z
• Authors: Sarasati Windria; Siti Isrina Oktav...; Widi Nugroho; Rini Widayanti; Soedarmanto Indar...
• Keywords: Haptoglobin; Streptococcus uberis; Somatic Cell Count; Streptococcus dysgalactiae; California mastitis test

The study described the development of a haptoglobin-based diagnostic tool for mastitis in Ettawa crossbreed goats. Fifty eight milk samples were collected from flock goats Yogyakarta, Central Java, Indonesia. All tested using California Mastitis Test (CMT), Somatic Cell Count (SCC), and Polymerase Chain Reaction (PCR) to identify Staphylococcus aureus, Streptococcus uberis agalactiae. presence haptoglobin mRNA proteins somatic cells was detected Sanger sequencing SDS-PAGE, respectively. Milk levels subsequently estimated an indirect enzyme-linked immunosorbent assay (ELISA) developed this study. Receiver Operating Characteristic (ROC) analysis performed compare sensitivity specificity CMT, SCC, ELISA PCR as reference standard. Kappa test used determine agreement between three imperfect tests. Results indicated that goat expressed with size 174 bp two molecular weights 18 kDa 32 kDa. showed 81% diagnosed positive mastitis. At level 50%, ROC more sensitive compared SCC or CMT (consecutively, 96%, 94%, 92%). values high (0.84 0.81, respectively). This indicates capable synthesizing secreting haptoglobin. can be potential target early detection