Embelin attenuates lipopolysaccharide-induced acute kidney injury through the inhibition of M1 macrophage activation and NF-κB signaling in mice

• DOI: 10.1016/j.heliyon.2023.e14006
• Publication Date: 2023-02-26T17:46:47Z
• Authors: Qiao Tang; Yun Tang; Qun Yang; Rong Chen; Hong Zhang; Haojun Luo; Qiong Xiao; Kaixiang Liu; Liming Huang; Jie Chen; Lin Wang; Xinrou Song; Sipei Chen; Guisen Li; Li Wang; Yi Li
• Keywords: Inflammation; H1-99; 0301 basic medicine; Science (General); Macrophage; Septic acute kidney injury; Embelin; 3. Good health; Social sciences (General); Q1-390; 03 medical and health sciences; Phosphorylated NF-κB p65 translocation; Research Article

Septic acute kidney injury (AKI) is commonly associated with renal dysfunction and high mortality in patients. Owing to the rapid violent occurrence of septic AKI inflammation, there are no effective therapies clinically treat it. Embelin, a natural product, has potential regulatory role immunocytes. However, mechanism embelin remains unknown. This study aimed elucidate macrophage regulation lipopolysaccharide (LPS)-induced AKI. Embelin was intraperitoneally administered mice after LPS injection. And bone marrow-derived macrophages (BMDMs) were subsequently isolated from explore immunomodulatory macrophages. We found that attenuated pathological damage LPS-induced sepsis mouse model. Molecular docking predicted could bind phosphorylated NF-κB p65 at ser536 site. inhibited translocation via phosphorylation It also reduced secretion IL-1β IL-6 increased IL-10 Arg-1 BMDMs stimulation, indicating suppressed M1 activation Therefore, by suppressing activated preclinically suggests therapeutic