Immune landscape and regulatory mechanisms in human atherosclerotic coronary plaques: Evidence from single-cell and bulk transcriptomics

• DOI: 10.1016/j.heliyon.2023.e19392
• Publication Date: 2023-08-23T01:12:44Z
• Authors: Wei-Lin Liang; Hui-Ling Liao; Bo Liang
• Keywords: Monocyte; Cell type

Atherosclerosis is a chronic immuno-inflammatory disease, however, the immune landscape and regulatory mechanisms have not been clear. We detected seven principal cell clusters with distinct phenotypic spatial characteristics using single-cell RNA-sequencing of aortic cells from patients acute coronary syndrome stable angina pectoris. Then we acquired 265 differentially expressed immune-related genes high scores were mainly found in T monocytes, which regulated atherosclerotic plaques. The CCL signaling pathway was most relevant pattern CCL5-CCR1 CCL5-CCR5 ligand-receptor pairs played vital role pathway. Further comparative analysis indicated MCH-I HLA ligand-related role. Functional bulk transcriptomics pointed to multiple pathways, such as antigen presentation response. Nineteen common both human peripheral blood mononuclear cells. Nine transcription factors monocyte plaques network demonstrated that CEBPB might play an essential transcriptional regulation atherosclerosis hub factor. definition diversity heterogeneity by level subsets only unveils cell-type-specific pathways new but also discovers functional correlation atherosclerosis. Our findings provide great promise for discovery novel molecular precise therapeutic targets