Antihypertensive potential of the stem bark of Canarium schweinfurthii Engl. (Burseraceae) in wistar rats: UPLC-ESI-QToF-MS/MS-based prediction of antihypertensive phytochemicals

• DOI: 10.1016/j.heliyon.2023.e21841
• Publication Date: 2023-11-04T17:31:47Z
• Authors: Jean Emmanuel Mbo...; Edwige Laure Nguemfo; Thierry Fokou Nzo...; Calvin Bogning Za...; Jarmo-Charles Kal...; Bienvenu Tsakem; Jules Clement Ass...; Xavier Siwe Noundou
• Keywords: Burseraceae

Ethnopharmacological relevanceCanarium schweinfurthii, also called ''Elemierd'Afrique'', is used in Cameroonian folk medicine (bark decoction) to treat patients suffering from hypertension.Aim of the study: This study aimed at evaluating antihypertensive activities stem bark Canarium schweinfurthii and identifying potential compounds present its extract that may support or oppose ethnomedicinial use.Materials methodsStem was prepared by maceration using 70 % ethanol followed redissolution methanol hyphenated. Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) analysis for detection characterisation secondary metabolites. Antihypertensive effects were assessed Wistar rats after induction hypertension with sodium chloride (NaCl) 18 a dose 0.01mL/gbody weight once day four weeks.Hemodynamic parameters measured weekly anon-invasive method CODA system.ResultsThe ethanolic C. significantly inhibited increase blood pressure maximum 23.18 (systolic pressure, p < 0.0001), 24.77 (diastolic 0.001) 22.95 (mean 0.0001) 200 mg/kgbody 4th week, compared agroup received only NaCl (negative control). Similarly, heart rate 18.84 (p week four. Hematological did not differ between extract-treated control groups. The UPLC-MS/MS spectrometric provided evidence presence several C30 terpenoids containing three five oxygen atoms exhibiting pentacyclic triterpenoid structures, as well C29 related nitrogen addition oxygen, spectral matching, silico molecular formula structure prediction. Additionally, two features annotated high-confidence lignans, structurally closely hinokinin dehydrocubebin through MS/MS-based prediction CSI: Finger ID SIRIUS5. lignans have been previously reported plants belonging Burseraceae family. Conclusion: demonstrated properties on tested rats. These results ethnopharmacological use concoctions treatment suggest protective effect against salt damage, hypothetically up regulation antioxidative enzymes and/or lipids, mitigatings membrane peroxidation.