Optimizing the formulation of Erwinia bacteriophages for improved UV stability and adsorption on apple leaves

• DOI: 10.1016/j.heliyon.2023.e22034
• Publication Date: 2023-11-08T09:59:08Z
• Authors: Su Jin Jo; Sang Guen Kim; Jungkum Park; Young Min Lee; Sib Sankar Giri; Sung Bin Lee; Won Joon Jung; Mae Hyun Hwang; Jae Hong Park; Eunjung Roh; Se Chang Park
• Keywords: Fire blight

Fire blight is a bacterial disease that affects plants of the Rosaceae family and causes significant economic losses worldwide. Although antibiotics have been used to control disease, concerns about their environmental impact potential promote antibiotic resistance arisen. Bacteriophages are being investigated as an alternative antibiotics; however, efficacy can be affected by stresses, such UV radiation. In this study, we optimized formulation Erwinia phages enhance stability in field, focusing on improving adsorption using adjuvants. Our results confirmed 4.5 % polysorbate 80 kaolin improve phage under stress, resulting increase PFU value improved protection efficacy. Adsorption assays also demonstrated absorption efficiency, with detected plant for up two weeks. These findings demonstrate effectiveness auxiliary bacteriophages against stress.