Effect of acupuncture therapy on vaccine-induced immune response in d-galactose-induced aging rats

• DOI: 10.1016/j.heliyon.2023.e22645
• Publication Date: 2023-11-19T03:29:06Z
• Authors: Jia Li; Fangyuan Liang; Ling Xiao; Wei Lu; Hua Wang
• Keywords: Social sciences (General); H1-99; 0301 basic medicine; Q1-390; 03 medical and health sciences; Science (General); HSP70 mRNA; Acupuncture; Immune response; Adjuvant; Research Article

ObjectiveThis study aimed to explore whether acupuncture and moxibustion can enhance the immune response by increasing expression of endogenous adjuvant HSP70 mRNA.MethodForty Wistar rats were divided into four groups: model group (A), control (B), normal (C), (D). Model groups A B induced injecting d-galactose for 6 weeks. Rats in C then treated with low-frequency electroacupuncture (EA) at Zusanli (ST36), Guanyuan (CV4), Baihui (GV20) 3 Subsequently, all observed 2 more At 12th week, diphtheria antitoxin titers determined using Vero cell trace neutralization method, CD4+T/CD8+T ratios peripheral blood examined flow cytometry, relative spleen mRNA was measured RT-PCR.ResultsCompared control, titer, ratio, significantly decreased (P < 0.01). However, showed a significant increase titer 0.01) elevated ratio 0.05) after EA intervention.ConclusionAcupuncture may humoral (diphtheria titer) cellular (peripheral ratio) mRNA, suggesting that serve as new vaccine adjuvant.