Antibacterial and osteoinductive properties of wollastonite scaffolds impregnated with propolis produced by additive manufacturing

• DOI: 10.1016/j.heliyon.2023.e23955
• Publication Date: 2023-12-19T01:15:21Z
• Authors: Ana Isabel Moreno...; Sarita Malagon; Sebastian Ocampo; Sara Leal-Marin; Jesús Humberto Gi...; Andres Diaz-Cano; Alex Lopera; Carlos Paucar; Alex Ossa; Birgit Glasmacher; Alejandro Peláez-...; Claudia Garcia
• Keywords: Propolis; Biocompatibility; Wollastonite; Gyroid

Biocompatible ceramic scaffolds offer a promising approach to address the challenges in bone reconstruction. Wollastonite, well-known for its exceptional biocompatibility, has attracted significant attention orthopedics and craniofacial fields. However, antimicrobial properties of wollastonite have contradictory findings, necessitating further research enhance antibacterial characteristics. This study aimed explore new improve vitro biological response terms activity cell proliferation by taking advantage additive manufacturing development with complex geometries 3D printing using propolis-modified wollastonite. The were designed TPMS (Triply Periodic Minimal Surface) gyroid geometric shape printed prior impregnation propolis extract. paste formulation was characterized rheometric measurements, presence confirmed FTIR spectroscopy. comprehensively assessed their mechanical strength. characterization involved evaluating effects against Staphylococcus aureus epidermidis, employing Minimum Inhibitory Concentration (MIC), Zone Inhibition (ZOI), biofilm formation assays. Additionally, SaOs-2 cultures used (Alamar blue assay), potential osteogenic tested (von Kossa, Alizarin Red, ALP stainings) at different time points. Propolis did not compromise scaffolds, which exhibited values comparable human trabecular bone. incorporation conferred both epidermidis. implementation geometry scaffold design demonstrated favorable increased metabolic after 21 days cultures.