A scalable route to quaternary ammonium-functionalized AgCl colloidal antimicrobials inhibiting food pathogenic bacteria and biofilms

• DOI: 10.1016/j.heliyon.2024.e25260
• Publication Date: 2024-02-01T17:10:11Z
• Authors: Diellza Bajrami; Syed Imdadul Hossain; Alexia Barbarossa; Maria Chiara Spor...; Rosaria Anna Picca; Luigi Gentile; Francesco Mastrol...; Antonio Rosato; Alessia Carocci; Nicola Antonio Co...; Boris Mizaikoff; Nicola Cioffi
• Keywords: Pathogenic bacteria; Zeta potential

This study explores how a simple argentometric titration-like approach could be evolved into versatile, scalable, fast, and robust strategy for the production of AgCl/quaternary ammonium compounds (QACs) colloidal nanoantimicrobials (NAMs). These systems, which are green, stable, cost-effective, reproducible found to effective against wide range food pathogenic bacteria biofilms. The option large-scale such suspensions was explored via use peristaltic pump. various types biosafe QACs solvents including aqueous organic ones renders this system green versatile. Nanocolloids were characterized using UV–Vis, X-ray photoelectron Fourier transform infrared (FTIR) spectroscopies. Their morphology crystalline nature investigated by transmission electron microscopy (TEM) selected area diffraction pattern (SAED). Nanoparticle (NP) size distribution hydrodynamic radius measured dynamic light scattering (DLS), while ζ-potential highly positive, thus indicating significant stability antimicrobial activity. In fact, higher NP surface charge, stronger their bioactivity. Furthermore, antibacterial antibiofilm effects as-prepared NCs tested Gram-positive bacteria, as Staphylococcus aureus (ATCC 29213) Listeria monocytogenes, Gram-negative Escherichia coli 25922) Pseudomonas aeruginosa 27853). results clearly indicate that AgCl/QACs provide pronounced activity with long-term bacteriostatic foodborne rendering them an ideal choice active packaging systems.