IL-22 promotes liver regeneration after portal vein ligation

• DOI: 10.1016/j.heliyon.2024.e27578
• Publication Date: 2024-03-08T21:54:16Z
• Authors: Tao Zhang; Philipp Seeger; Yashin Simsek; Morsal Sabihi; Jöran Lücke; Dimitra E. Zazara; Ahmad Mustafa Shiri; Jan Kempski; Tom Blankenburg; Lilan Zhao; Ioannis Belios; Andres Machicote; Baris Mercanoglu; Mohammad Fard-Aghaie; Sara Notz; Panagis M. Lykoudis; Marius Kemper; Tarik Ghadban; Oliver Mann; Thilo Hackert; Jakob R. Izbicki; Thomas Renné; Samuel Huber; Anastasios D. Gia...; Jun Li
• Keywords: Liver Regeneration

BackgroundInsufficient remnant liver volume (RLV) after the resection of hepatic malignancy could lead to failure and mortality. Portal vein ligation (PVL) prior hepatectomy is subsequently introduced increase improve outcome malignancy. IL-22 has previously been reported promote regeneration, while facilitating tumor development in via Steap4 upregulation. Here we performed PVL mouse models study role regeneration post-PVL.MethodsLiver weight was measured magnetic resonance imaging (MRI). Immunohistochemistry for Ki67 hepatocyte growth factor (HGF) performed. analyzed by flow cytometry quantitative polymerase chain reaction (qPCR) used acquisition Il-33, Steap4, Fga, Fgb Cebpd. To analyze signaling pathways, mice with deletion STAT3 a neutralizing antibody were used.ResultsThe increased over time PVL. Additionally, found that regenerative molecules, including HGF, significantly at day 3 post-PVL, as well IL-22. Administration reduce expression The upregulation mainly derived from innate cells. blockade resulted lower levels IL-33 liver, which also case STAT3, main downstream molecule IL-22, hepatocytes.ConclusionIL-22 promotes Thus, combination supplementation potentially be applied novel therapeutic approach boost without progression