Assessment of the feasibility of human amniotic membrane stem cell-derived cardiomyocytes in vitro

• DOI: 10.1016/j.heliyon.2024.e28398
• Publication Date: 2024-03-19T08:21:50Z
• Authors: Hsiu-Man Shih; Yi-Chen Chen; Yen-Ting Yeh; Fu-Shiang Peng; Shinn-Chih Wu
• Keywords: CD90; Amniotic stem cells; Amniotic epithelial cells; Cell therapy

Myocardial infarction (MI) is a leading cause of death worldwide, resulting in extensive loss cardiomyocytes and subsequent heart failure. Inducing cardiac differentiation stem cells potential approach for myocardial regeneration therapy to improve post-MI prognosis. Mesenchymal (MSCs) have several advantages, including immune privilege multipotent potential. This study aimed explore the feasibility chemically inducing human amniotic membrane MSCs (hAMSCs) differentiate into vitro. Human (AM) samples were obtained from routine caesarean sections at Far Eastern Memorial Hospital. The isolated exhibited spindle-shaped morphology expressed surface antigens CD73, CD90, CD105, CD44, while lacking expression CD19, CD11b, CD45, HLA-DR. SSEA-1, SSEA-3, SSEA-4 markers also positive, displayed ability tri-lineage adipocytes, chondrocytes, osteoblasts. levels MLC2v, Nkx2.5, MyoD analyzed using qPCR after applying various protocols chemical induction, BMP4, ActivinA, 5-azacytidine, CHIR99021, IWP2 on hAMSCs. group treated with 5 ng/ml 10 Activin A, μM 7.5 IWP 2 highest these genes. Furthermore, immunofluorescence staining demonstrated α-actinin Troponin T this group. In conclusion, that hAMSCs can be induced cardiomyocyte-like However, functionality differentiated cells, further investigation inductive regimens needed.