Exosomal miR-151-3p in saliva: A potential non-invasive marker for gastric cancer diagnosis and prognosis modulated by Sijunzi decoction (SJZD) in mice

• DOI: 10.1016/j.heliyon.2024.e29169
• Publication Date: 2024-04-03T23:53:00Z
• Authors: Ping Yang; Huijun Lei; Yue Fu; Cheng Chen; Li Tang; Shuaishuai Xia; Yan Guo; Guangyu Chen; Mengzhou Xie; Jingjing Yang; Feng Li; Liang Li
• Keywords: KEGG; Exosome; Decoction

Gastric cancer (GC) is one of the most prominent malignancies that originate in epithelial cells gastric mucosa and main causes cancer-related mortality worldwide. New circulating biomarkers exosomal RNA might have great potential for non-invasive early prognosis GC. Sijunzi Decoction (SJZD) a typical representative formula method benefiting Qi strengthening spleen Traditional Chinese Medicine (TCM). However, effects mechanism SJZD treating GC remain unclear. This study looked GC, explored A model with deficiency syndrome was established nude mice, curative were investigated. Differentially expressed miRNAs plasma saliva exosomes sequenced analyzed. Potential target genes these predicted applied Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) signaling pathway enrichment annotation. Overlapping samples analyzed, qRT-PCR performed verification. miR-151a-3p selected, further determined downregulated from group. The intersected enriched extrinsic apoptotic pathways. significantly ameliorates mouse models, miRNAs, particularly miR-151-3p, be modulated by saliva. miR-151-3p may serve as marker diagnosis prognosis.