High-yield fabrication of monodisperse multilayer nanofibrous microparticles for advanced oral drug delivery applications

• DOI: 10.1016/j.heliyon.2024.e30844
• Publication Date: 2024-05-08T02:28:19Z
• Authors: Fatemeh Ajalloueian; Lasse Højlund Ekl...; Chiara Mazzoni; Ritika Singh Pete...; Stephan Sylvest K...; Anja Boisen
• Keywords: Dispersity; Electrospinning; PLGA; Pullulan; Microparticle

Recent advances in the use of nano- and microparticles drug delivery, cell therapy, tissue engineering have led to increasing attention towards nanostructured microparticulate formulations for maximum benefit from both micron sized features. Scalable manufacturing monodisperse with tunable size, shape, content, release rate remains a big challenge. Current technology, mainly comprises complex multi-step chemical procedures limited control over these aspects. Here, we demonstrate novel technique high-yield fabrication monolayer multilayer nanofibrous (MoNami MuNaMi respectively). The procedure includes sequential electrospinning followed by micro-cutting at room temperature transfer particles collection. advantage introduced is potential apply several polymer-drug combinations forming enjoying extracellular matrix (ECM)-mimicking architecture profile. We study factors affecting final three-dimensional structure. A model encapsulated into three-layer sheet (PLGA-pullulan-PLGA), how profile changes burst sustain simply cutting out electrospun sheet. believe our method offers unique facile platform realizing advanced oral delivery applications.