Sustained feeding of a diet high in fat resulted in a decline in the liver's insulin-degrading enzyme levels in association with the induction of oxidative and endoplasmic reticulum stress in adult male rats: Evaluation of 4-phenylbutyric acid

• DOI: 10.1016/j.heliyon.2024.e32804
• Publication Date: 2024-06-13T00:41:11Z
• Authors: Fateme Binayi; Behnam Saeidi; Fatemeh Farahani; Mina Sadat Izadi; Farzaneh Eskandari; Fariba Azarkish; Mohammad Sahraei; Rasoul Ghasemi; Fariba Khodagholi; Homeira Zardooz
• Keywords: Malondialdehyde

The current study explored the impact of high fat diet (HFD) on hepatic oxidative and endoplasmic reticulum (ER) stress its insulin degrading enzyme (IDE) content with injection 4-phenyl butyric acid (4-PBA) in adult male rats.Following weaning period, offspring were distributed among six distinct groups. corresponding was used for 20 weeks, subsequently 4-PBA administered three consecutive days. Plasma glucose levels, HOMA-β (homeostasis model assessment β-cell), ER biomarkers IDE protein assessed.Long-term ingestion HFD (31 % cow butter) induced liver tissue. Accordingly, a rise malondialdehyde (MDA) catalase activity decrease glutathione (GSH) detected within + DMSO Consumption this elevated expression binding immunoglobulin (BIP) C/enhancer-binding homologous (CHOP) levels while reduced content. decreased significantly. moderated all changes.Findings from indicated that prolonged consumption led to reduction plasma likely attributed pancreatic β cell malfunction, as evidenced by decline index. Also, appears have triggered liver, along