Reprogramming human urine cells into intestinal organoids with long-term expansion ability and barrier function

• DOI: 10.1016/j.heliyon.2024.e33736
• Publication Date: 2024-06-27T07:57:22Z
• Authors: Ruifang Zhang; Yating Chen; Ziyu Feng; Baomei Cai; Yiyi Cheng; Yunjing Du; Sihua Ou; Huan Chen; Mengjie Pan; He Liu; Duanqing Pei; Shangtao Cao
• Keywords: Organoid; Reprogramming

Generation of intestinal organoids from human somatic cells by reprogramming would enable regeneration, disease modeling, and drug screening in a personalized pattern. Here, we report direct protocol for the generation urine induced (U-iIOs) under defined medium. U-iIOs expressed multiple specific genes showed resembling gene expression profiles to primary small intestines. can be stably long-term expanded further differentiated into more mature lineage with high metallothionein cytochrome P450 (CYP450) genes. These molecular features differ pluripotent stem derived (P-iIOs) immortalized cell lines. Furthermore, exhibit barriers indicated blocking FITC-dextran permeation uptaking substrate rhodamine 123. Our study provides novel platform patient-specific organoid generation, which may lead precision treatment diseases facilitate discovery.