Anti-angiogenic activity of a novel angiostatin-like plasminogen fragment produced by a bacterial metalloproteinase

• DOI: 10.1016/j.heliyon.2024.e35232
• Publication Date: 2024-07-27T00:42:38Z
• Authors: Kosuke Shimizu; Naoko Nishimura; Taolin Wang; Tetsuro Yamamoto; Eriko Suzuki; Keiji Hasumi
• Keywords: Angiostatin; Endostatin; Fragment (logic)

Tumor growth depends on angiogenesis, a process by which new blood vessel are formed from pre-existing normal vessels. Proteolytic fragments of plasminogen, containing varying numbers plasminogen kringle domains, collectively known as angiostatin, naturally occurring inhibitor angiogenesis and inhibit tumor growth. We have developed an "affinity-capture reactor" that enables single-step method for the production/purification angiostatin-like fragment human plasma using immobilized bacterial metalloproteinase. The resulting fragment, named BL-angiostatin, contains one or two glycosyl chains N-terminal PAN module, not present in canonical angiostatins tested cancer treatment. BL-angiostatin inhibited