Clinical diagnostic value of metagenomic next-generation sequencing in patients with acute infection in emergency department

• DOI: 10.1016/j.heliyon.2024.e35802
• Publication Date: 2024-08-08T14:11:13Z
• Authors: Lingyu Wei; Jieyu Luo; Weiwei Wu; Jia Yin; Zaiyuan Sun; Xue Xu; Wenqian Gong; Jia Xu
• Keywords: Clinical Microbiology

ObjectiveTo explore the value of metagenomic next-generation sequencing (mNGS) and culture in microbial diagnosis patients with acute infection.MethodsWe retrospectively analyzed 206 specimens from 163 who were admitted to emergency department The First Affiliated Hospital Sun Yat-sen University between July 2020, 2021. We evaluated diagnostic efficacy mNGS in-hospital traditional culture.ResultsThe total positive rate was significantly higher than that methods (71.4 % vs 40.8 %, p < 0.001), while sensitivity accuracy found be 92.9 88.2 respectively. However, exhibited superior specificity a 92.6 compared 75.9 for mNGS. detection efficiency fungi comparable, but showed performance bacterial detection. In analysis sepsis samples, outperformed diagnosing various types especially sputum bronchoalveolar lavage fluid. Among identified infections, infections most common single infection (37.5 %). Additionally, bacterial-fungal represented prevalent form mixed (77.3 Candida albicans Staphylococcus aureus as predominant pathogens survival death groups, No significant differences diversity observed.ConclusionCompared methods, demonstrates rates, sensitivity, rapid particularly critically ill such those sepsis. This capability establishes foundation swift precise identification pathogens, allowing clinical indicators patient prognosis based on extensive data generated