HPLC-DAD method for simultaneous determination of gallic acid, catechins, and methylxanthines and its application in quantitative analysis and origin identification of green tea

• DOI: 10.1016/j.heliyon.2024.e35819
• Publication Date: 2024-08-06T16:53:17Z
• Authors: Silvia Jakabová; Július Árvay; Marek Šnirc; Jana Lakatošová; Alena Ondejčíková; Jozef Golian
• Keywords: Theobromine; Quantitative Analysis

The high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD) was optimized for the simultaneous determination of 11 compounds, belonging to polyphenols (gallic acid and seven catechins) methylxanthines (caffeine, theobromine, theophylline). results obtained all validation parameters HPLC-DAD method showed that is sensitive enough routine analysis basic chromatographic equipment, thus it has significant potential be highly applicable in common laboratory practice. used 60 green tea infusions originating from four tea-producing countries. dataset contributes enhancing current data on tea. extracts revealed differences depending origin samples. Linear Discriminant Analysis (LDA) applied test accuracy identification samples, based chemical composition focus polyphenolic compounds analysed this study. Based cross-validation results, model 93.75 % classification Japan, China (Mainland), (Taiwan) South Korea.