Huaier relieves oxaliplatin-induced hepatotoxicity through activation of the PI3K/AKT/Nrf2 signaling pathway in C57BL/6 mice

• DOI: 10.1016/j.heliyon.2024.e37010
• Publication Date: 2024-08-29T00:46:51Z
• Authors: Xinwei Cheng; Chen Zhu; Yunzhou Chen; Min Li; Guodong Li; Yue Zu; Qianyan Gao; Tianze Shang; Dong Liu; Chengliang Zhang; Xiuhua Ren
• Keywords: Malondialdehyde

Highlights•Huaier can be an effective drug to reduce oxaliplatin-induced hepatotoxicity.•ROS-mediated oxidative stress is involved in hepatotoxicity.•Huaier relieves liver stress.•Inhibition of PI3K pathway reverses the hepatoprotective effect Huaier.AbstractHepatotoxicity caused by anticancer medication oxaliplatin (OXA) significantly restricts its clinical use and raises risk damage. Huaier, a fungus found China, has been demonstrated have various beneficial effects adjuvant therapy for cancer. However, preventive impact Huaier against OXA-induced hepatotoxicity still unknown. The potential molecular pathways behind activity were investigated current study Mice intraperitoneally injected with 10 mg/kg OXA once week six consecutive weeks establish injury model. (2 g/kg, 4 8 g/kg) was administered weekly mice gavage weeks. Commercial kits used determine contents glutathione, catalase, superoxide dismutase, malondialdehyde. Quantitative real-time polymerase chain reaction (qRT-PCR) Western blotting assess on expression pathway. exhibited good protective dose-dependent manner, which connected suppression stress, according results biochemical index detection histological staining analysis. In addition, could counteract PI3K/AKT signaling Moreover, activation eradicated LY294002. These findings imply that decreasing minimize injury, establishing groundwork lessen chemotherapy-induced practice.