Co-metabolic degradation of pyrene by indigenous white-rot fungus Pseudotrametes gibbosa from the northeast China

• DOI: 10.1016/j.ibiod.2011.03.003
• Publication Date: 2011-04-20T23:52:01Z
• Authors: Jiwei Wen; Dawen Gao; Bo Zhang; Hong Liang
• Keywords: Phthalic acid

Abstract Pyrene as high molecular weight polycyclic aromatic hydrocarbons (PAHs) is found in most terrestrial environment. However, the biodegradation of pyrene has been limited due to its bioavailability and toxicity. So it is vital to study degradation-capable microorganism and suitable co-substrate. In this study, the indigenous white-rot fungus Pseudotrametes gibbosa isolated from ChangBai Mountain located in northeast China was used to degrade pyrene, and 6 co-substrates were selected as co-metabolic carbon and energy sources. The results showed that P. gibbosa was able to utilize pyrene as sole carbon and energy source. The degradation efficiency achieved 28.33% within 18 days. Meanwhile, co-substrate wheat bran extract could stimulate laccase (Lac) production significantly by P. gibbosa , compared with other co-substrates and control (without co-substrate). In the presence of co-substrates, the biodegradation efficiency of pyrene ranged from 34.23% to 50.64% which was enhanced due to co-metabolism except for salicylic acid (25.91%) and phthalic acid (21.64%).