A novel role of Drosophila cytochrome P450-4e3 in permethrin insecticide tolerance

• DOI: 10.1016/j.ibmb.2015.06.002
• Publication Date: 2015-06-14T02:49:22Z
• Authors: Selim Terhzaz; Pablo Cabrero; Robert A. Brinzer; Kenneth A. Halberg; Julian A.T. Dow; Shireen-A. Davies
• Keywords: Malpighian tubule system; CYP2B6

The exposure of insects to xenobiotics, such as insecticides, triggers a complex defence response necessary for survival. This includes the induction genes that encode key Cytochrome P450 monooxygenase detoxification enzymes. Drosophila melanogaster Malpighian (renal) tubules are critical organs in and elimination these foreign compounds, so tubule induced by dietary insecticide permethrin was examined. We found expression gene encoding P450-4e3 (Cyp4e3) is significantly up-regulated fed on manipulation Cyp4e3 levels, specifically principal cells tubules, impacts survival permethrin-fed flies. Both knockdown cause significant elevation oxidative stress-associated markers including H2O2 lipid peroxidation byproduct, HNE (4-hydroxynonenal). Thus, may play an important role regulating levels endoplasmic reticulum (ER) where it resides, its absence JAK/STAT NF-κB-mediated stress response, similar observed under ER stress. work increases our understanding molecular mechanisms provides further evidence responses metabolism.