Lipidome remodeling in aging normal and genetically obese Drosophila males

• DOI: 10.1016/j.ibmb.2020.103498
• Publication Date: 2020-11-20T13:39:35Z
• Authors: Harald F. Hofbauer; Christoph Heier; Anantha Krishnan ...; Ronald P. Kühnlein
• Keywords: Lipidome; Lipid droplet

Lipid homeostasis is essential for insects to maintain phospholipid (PL)-based membrane integrity and provide on-demand energy supply throughout life. Triacylglycerol (TAG) the major lipid class used production stored in droplets, universal cellular fat storage organelles. Accumulation mobilization of TAG are strictly regulated since excessive accumulation leads obesity has been correlated with adverse effects on health- lifespan across phyla. Little known, however, about when during adult life why restricts lifespan. We here genetically obese Drosophila mutant males, which were all shown be short-lived compared control males applied single fly mass spectrometry-based lipidomics profile TAG, diacylglycerol signatures from eclosion death. Our comparative approach revealed distinct phases lipidome remodeling aging. Quantitative qualitative compositional changes PL species, characterized by length saturation their constituent fatty acids, pronounced young In contrast, senescent flies remarkably stable. Genetically displayed both quantitative species composition, while almost unaltered normal at ages. Collectively, this suggests a tight composition lifetime largely uncoupled metabolism. Finally, we present first evidence characteristic signature moribund flies, likely generated rapid selective depletion close Of note, analytical power monitor profiles combined high sensitivity universally applicable address developmental or behavioral modulations importance insect