AmpFℓSTR®Y-filer™: A new tool for rapid Y-STR forensic haplotyping

• DOI: 10.1016/j.ics.2005.09.132
• Publication Date: 2006-04-21T21:37:53Z
• Authors: M.L. Pontes; D. Abrantes; G. Lima; L. Cainé; M.J. Pereira; P. Matos; M.F. Pinheiro
• Keywords: Y-STR; STR analysis; STR multiplex system

Abstract Allele frequencies and population data for 17 Y-STR loci, including all the markers in the actually used European “extended haplotype” (DYS19, DYS189I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385I/II, DYS438 and DYS439) and also DYS437, DYS448, DYS456, DYS458, DYS635 and Y GATA H4 included in a new commercial kit that has recently been available, the AmpFlSTR® Y-filer™ PCR amplification kit (Applied Biosystems), were obtained from a sample of 175 healthy unrelated males and 45 father–son pairs from the North of Portugal. A total of 171 haplotypes were identified, of which 167 were unique and 4 found in two individuals. The haplotype diversity (99. 97%) and discrimination capacity (95.43%) were calculated.