Berberine coated biocomposite hemostatic film based alginate as absorbable biomaterial for wound healing

• DOI: 10.1016/j.ijbiomac.2022.04.132
• Publication Date: 2022-04-26T15:22:02Z
• Authors: Haofeng Hu; Fulin Luo; Qian Zhang; Ming Xu; Xin Chen; Zhihao Liu; Haodong Xu; Lei Wang; Fei Ye; Kui Zhang; Bin Chen; Song Zheng; Jia Jin
• Keywords: Biomaterial; Biocompatibility; Biocomposite

In wound treatment, severe bleeding and infection are always primary challenges. Therefore, it is highly desired to develop novel dressing with both hemostatic and antibacterial capability. Herein, a series of biocomposite hemostatic films (BHFs) based alginate/chitosan/collagen-berberine have been prepared and well characterized for further biofunctional study. We have demonstrated that the hemostatic and antibacterial activities were significantly enhanced by calcium/berberine dual-crosslinking system in the film. Through the synergistic effects, BHF-6B exhibited a shorter in vivo clotting and wound healing time than that of commercial dressing in rat tail amputation and full-thickness skin defect models. Additionally, BHF-6B showed excellent bacteriostatic activity with long-term effects. Moreover, hemolysis and cytotoxicity tests in vitro illustrated the prominent biocompatibility of the composite films. Notably, BHF-6B could be degraded quickly and completely in vivo. Overall, the present work indicated that the functionalized BHF-6B has great potential as an absorbable biomaterial for wound treatment.