Olfactory ensheathing cells conditioned medium prevented apoptosis induced by 6‐OHDA in PC12 cells through modulation of intrinsic apoptotic pathways

• DOI: 10.1016/j.ijdevneu.2008.01.003
• Publication Date: 2008-01-22T14:58:40Z
• Authors: Lei Feng; Haiwei Meng; Fengxia Wu; Baohua Cheng; Xiuquan He; Xiaohong Wang; Zhengping Li; Shuwei Liu
• Keywords: Olfactory ensheathing glia; MTT assay

Abstract Olfactory ensheathing cells (OECs) express a high level of growth factors which play very important role as neuronal support. Recent evidence in literatures showed that transplantation OECs may improve functional restoration 6‐OHDA‐induced rat model Parkinson's disease (PD). However, the biological function various released from Parkinson' is still unclear. In this study, we examined effects newborn conditioned medium (CM) on PC12 cells. Cells treated with 6‐OHDA underwent cytotoxicity and apoptotic death determined by MTT assay Hoechst 33342/PI staining. CM was able to reduce cellular damage Further investigation results inhibited disruption mitochondrial transmembrane potential, up‐regulation Bcl‐2 down‐regulation Bax. Taken together, study indicates has neuroprotective effect induced apoptosis cells, through Bcl‐2/Bax ratio protection for mitochondrion.