Developmental changes in the mammalian gonadotropin‐inhibitory hormone (GnIH) ortholog RFamide‐related peptide (RFRP) and its cognate receptor GPR147 in the rat hypothalamus

• DOI: 10.1016/j.ijdevneu.2011.10.003
• Publication Date: 2011-10-28T22:40:41Z
• Authors: Takeshi Iwasa; Toshiya Matsuzaki; Masahiro Murakami; Riyo Kinouchi; Tomohiro Osugi; Ganbat Gereltsetseg; Shinobu Yoshida; Minoru Irahara; Kazuyoshi Tsutsui
• Keywords: Gonadotropin; Kisspeptin; Sex steroid; Hypothalamic–pituitary–gonadal axis

Abstract The mammalian gonadotropin‐inhibitory hormone (GnIH) ortholog RFamide‐related peptide (RFRP) is considered to act on gonadotropin‐releasing (GnRH) neurons and the pituitary inhibit gonadotropin release synthesis. To understand functional significance of this neuropeptide, we investigated physiological changes in RFRP at mRNA levels, as well level its cognate receptor, G protein‐coupled receptor 147 ( GPR147 ), rat hypothalamus during development. We also effects gonadal steroids expression levels these molecules. In male rats, expressions both increased from postnatal days 12 16, peaking 35 42, respectively. However, their fell day 49. female continued increase throughout development; rats 28, but decreased 35. hypothalamic contents 28 42 were significantly higher than 4 those females. Neither orchidectomy nor ovariectomy influenced or prepubertal period when endogenous sex steroid low males Administration estradiol‐17β (E2) These results suggest that system An ovarian steroid, E2, may stimulate basal E2 concentration low.