Phenotypic and genotypic characterization of antimicrobial resistance in Escherichia coli isolates

• DOI: 10.1016/j.ijid.2014.03.836
• Publication Date: 2014-05-27T17:19:45Z
• Authors: E. Igbinosa; I. Ogboghodo; I.S. Obuekwe; F.E. Oviasogie
• Keywords: Kanamycin

Background: The major influences on the increase and spread of antimicrobial resistant bacteria are use agents in human medicine their livestock for therapy growth promotion. To generate baseline data to future risk assessment resistance, a number surveillance program local, continental global scale have been initiated. prevalence resistance commensal E. coli is good indicator selective pressure by antibiotics problems be expected pathogenic bacteria. aim this study was determine compare occurrence phenotypes genotypes among strains recovered from animal sources. Methods & Materials: Fifty-eight (58) were isolated sources (Cattle, pig chicken). MIC determined according Clinical Laboratory Standards Institute (CLSI) guidelines. Strains screened mapped PCR methods genes, integrons gene cassettes. Results: profile ampicillin (100%), cefotaxime ceftazidime (83%), ciprofloxacin (43%) (C), tetracycline (100%) (T), gentamicin (90%) (G), kanamycin (77%) (K), streptomycin (S), chloramphenicol (97%) (Ch) sulphamethoxazole (Su) trimethoprime (T). Eight-Five per cent (85%) harbored class 1 integron variable cassettes revealed. A that present observed. genes determinant aac(3')-IIc, aac(6')-Ib ant(2")-1a. aph(3)-Ia 68%. sul1 (80%) sul2 tet(A) higher than tet(B) tetracycline-resistant strains. determinants (cat1 catB) detected. Differences rates observed, possibly reflecting differences antibiotic regimens these Conclusion: This shows multi-drug isolates prevalent considerable proportion variety agents. Genotypic characterization revealed high diverse determinants.