Contamination, antimicrobial resistant of Campylobacter jejuni and Campylobacter coli isolated from retail meats in Italy and comparison with human strains

• DOI: 10.1016/j.ijid.2018.11.197
• Publication Date: 2019-01-30T17:37:57Z
• Authors: G. Di Donato; R. Nuvoloni; F. Marotta; L. Di Marcantonio; S. Antoci; D. Neri; P. Calistri; E. Di Giannatale
• Keywords: Multilocus sequence typing; Campylobacter coli; Broth microdilution; Raw meat

Purpose: Campylobacter is an important zoonotic pathogen potentially causing illness in humans, following the consumption of raw/undercooked meat. The objectives this study were to determine prevalence spp. retail meat Italy and assess antimicrobial resistance genetic relatedness strains from human cases. Methods & Materials: A total 2,596 fresh samples (1,393 chicken 1,203 bovines) taken at randomly selected supermarket during 2016. Presence level contamination determined using ISO methods evaluated with microdilution method. Isolates typed by MLST: sequence types (STs), clonal complexes (CCs) assigned submitting DNA MLST database website. All isolates compared humans same period. Results: was found more frequently 223 out 1,393 (16.01%) than bovine 7 (0.58%), difference statistically significant, p < 0,0001. In meats identified 132 C. jejuni (59.19%) 91 coli (41.55%). 3 4 coli. 41.63% very low (1-9 cells), 57.93% 10-999 cfu/g only 0.44% >1000ufc/g. samples, levels constantly below 10 ufc/g. High ciprofloxacin, nalidixic acid, tetracicline multi-drug pattern CipNaTe CipNaETe observed most strains. typing 78 STs 17 CCs. prevalent CCs 828, followed 353,354,21 206. Many poultry samples. Conclusion: Our confirms that main source campylobacteriosis humans. red had a suggesting minor role transmission illness. Particularly worrying increase against fluoroquinolones. Moreover, our results on acid tetracycline agreed those EFSA Report other studies. molecular proved be helpful tool epidemiological investigations.