Evaluation of the BioFire FilmArray Pneumonia Panel for rapid detection of respiratory bacterial pathogens and antibiotic resistance genes in sputum and endotracheal aspirate specimens

• DOI: 10.1016/j.ijid.2020.03.024
• Publication Date: 2020-03-13T22:41:17Z
• Authors: In Young Yoo; Kyungmin Huh; Hyang Jin Shim; Sun Ae Yun; Yoo Na Chung; On Kyun Kang; Hee Jae Huh; Nam Yong Lee
• Keywords: Klebsiella pneumonia

ObjectivesThe performance of the investigational-use-only version BioFire FilmArray Pneumonia Panel (FA-Pneumo), a high-order nested multiplex PCR, was evaluated for detection typical respiratory bacterial pathogens and antibiotic resistance genes in sputa endotracheal aspirate (ETA) specimens.MethodsThirty-one 69 ETA specimens were analyzed. The diagnostic FA-Pneumo assessed using routine microbiological methods as reference standard.ResultsOverall sensitivity specificity organism 98.5% 76.5%, respectively. each pathogen 100%, except Klebsiella aerogenes, range 83.3–99.0%. detected antimicrobial 17 out 18 (94.4%) that resistant by susceptibility testing. additionally 25 22 specimens, sequencing presence confirmed majority these results (20/25, 80%). Semi-quantitative analysis nucleic acid amounts revealed 88.2% identified bacteria (67/76) with ≥106 copies/ml also gave culture-positive significant bacteria.ConclusionsFA-Pneumo is rapid test high sputum could aid determining therapy.