Mutations in the cueA gene encoding a copper homeostasis P-type ATPase reduce the pathogenicity of Pseudomonas aeruginosa in mice

• DOI: 10.1016/j.ijmm.2005.05.005
• Publication Date: 2005-08-18T11:46:55Z
• Authors: William R. Schwan; Paul Warrener; Elizabeth Keunz; C. Kendall Stover; Kim R. Folger
• Keywords: Wild type; Pseudomonas putida

A sequencing project identified a putative copper homeostasis gene, cueA, in Pseudomonas aeruginosa strain PAO1. Strains with mutations of the cueA encoding P-type ATPase linked to P. putida, displayed greater sensitivity compared wild-type bacteria using MIC determinations and vitro passage growth media different concentrations added. An LD50 assay showed deletion mutant was 50-fold more attenuated than PAO1 bacteria. Complementation mutation restored tolerance virulence systemic model infection near levels. Competition assays between mutants strains demonstrated 20-fold attenuation by within spleens mice. This data suggests CueA protein may be important maintaining both vivo.