Evaluation of an in-house IgM/IgG lateral flow assay for serodiagnosis of human brucellosis

• DOI: 10.1016/j.ijmmb.2023.01.006
• Publication Date: 2023-02-03T18:26:35Z
• Authors: Rajeswari Shome; Sudipta Patra; Muneera Mohamed S...; G. Shanmugam; Shiva Dubey; Somy Skariah; Samer Shamshad; Nagendra Nath Barman; Durlav Prasad Bora; Arijit Shome; Nagalingam Mohandoss; Bibek Ranjan Shome
• Keywords: Point-of-Care Testing; Point of care

This study aimed to evaluate the diagnostic efficacy of an in-house lateral flow assay (LFA) for detection IgM/IgG anti-Brucella antibodies rapid serodiagnosis human brucellosis. Three groups sera samples including 476 from high-risk individuals, 27 culture-confirmed patients, and 43 healthy blood donors were used evaluation LFA. In comparison with iELISA, sensitivity, specificity, accuracy LFA >95%, >99%, 99% respectively. Considering very good agreement, accuracy, simplicity, rapidity, LFAs might be useful as a point care test diagnosis brucellosis in resource-limited laboratories.