Evaluation of the GenoType MTBDRplus assay for detection of rifampicin- and isoniazid-resistant Mycobacterium tuberculosis isolates in central Ethiopia

• DOI: 10.1016/j.ijmyco.2016.06.005
• Publication Date: 2016-06-27T14:47:03Z
• Authors: Zufan Bedewi Omer; Yalemtsehay Mekonnen; Adane Worku; Aboma Zewde; Girmay Medhin; Temesgen Mohammed; Rembert Pieper; Gobena Ameni
• Keywords: Gold standard (test); Mycobacterium tuberculosis complex

<br><b><i>Objective/Background:</i></b> Multidrug-resistant tuberculosis (MDR-TB) is growing globally and becoming a major challenge for national TB control programs. Therefore, rapid identification of MDR strains <i>Mycobacterium tuberculosis</i> monitoring their transmission could contribute significantly to the TB. The GenoType MTBDR<i>plus</i> assay has been recommended by World Health Organization identify rifampicin (RIF)- isoniazid (INH)-resistant <i>M. Tuberculosis</i> isolates. This study was carried out evaluate performance detection RIF- INH-resistant isolates in central Ethiopia. <b><i>Methods:</i></b> A total 279 isolated from active cases Ethiopia were evaluated drug sensitivity conventional drug-susceptibility test (DST) compared with data derived assay. DST served as gold standard evaluating <b><i>Results:</i></b> specificity RIF-resistant 80.0% 99.6%, respectively. Its 82.7% respectively, whereas they 75.0% 100%, strains. concordances RIF INH susceptibility 80% (8/10) 86.2% (25/29), Furthermore, concordance two tests 75%. Specific mutations detected 55.6% (5/9) isolates, highest mutation rate (33.3%) <i>rpoB</i> gene (Codon S531L). For (88.8%) related <i>katG</i> S315T1). <b><i>Conclusion:</i></b> findings this revealed that high resistance. These preliminary support notion should be considered an alternative characterization TB.<br>