Uncovering Trypanosoma spp. diversity of wild mammals by the use of DNA from blood clots

• DOI: 10.1016/j.ijppaw.2019.02.004
• Publication Date: 2019-02-14T17:30:12Z
• Authors: Marina Silva Rodr...; Luciana Lima; Samanta Cristina ...; Heitor Miraglia H...; Fabiana Lopes Rocha; André Luiz Rodrig...; Marta Maria Geral...; Ana Maria Jansen
• Keywords: Trypanosomatida; Trypanosomatidae; Euglenozoa; bats; bat; Biodiversity; Article; 3. Good health; 03 medical and health sciences; 0302 clinical medicine; QL1-991; Chiroptera; Mammalia; Animalia; Kinetoplastea; Protozoa; Chordata; Zoology; Taxonomy

Trypanosoma spp. infection in wild mammals is detected mainly through parasitological tests that usually display low sensitivity. We propose the use of DNA extracted directly from blood clots (BC), which are neglected sources for diagnosis and identification This approach followed by nested PCR targeting 18S SSU rDNA demonstrated to be sensitive suitable evaluate diversity trypanosomes infecting sylvatic mammals, including subpatent mixed infections. Infection was 95/120 (79.2%) samples bats, carnivores marsupials included negative serological hemoculture testing mammals. Thirteen or Molecular Operational Taxonomic Units (MOTUs) were identified, two new MOTUs. The high species MOTUs bats showed these hosts can considered as bio-accumulators spp., with specimens Didelphis displaying highest trypanosome diversity. allowed direct access non-culturable parasites, infections, besides bypassing selective pressure on parasites inherent cultivation procedures. cruzi found number individuals, T. lainsoni. Positive observed 16 seronegative individuals 30 hemocultures. Also, lainsoni, previously only rodents, capable marsupials. finding makes it clear some more generalist than thought. using BC increase knowledge about host-spectrum distribution