Trypanosoma spp. Neobats: Insights about those poorly known trypanosomatids

• DOI: 10.1016/j.ijppaw.2021.09.003
• Publication Date: 2021-09-10T15:18:05Z
• Authors: Fernanda Moreira ...; Diana Azeredo Rangel; Emmanuel Messias ...; Márcio Galvão Pavan; Ricardo Moratelli; André Luiz Rodrig...; Ana Maria Jansen
• Keywords: Trypanosoma; 0303 health sciences; bats; Blood clot; bat; Biodiversity; Article; 03 medical and health sciences; Neobat; QL1-991; Crithidia; Chiroptera; Bats; Mammalia; Animalia; MOTU; Chordata; Zoology; Taxonomy

Bats are infected with several trypanosomatid species; however, assessing the diversity of this interaction remains challenging since there species apparently unable to grow in conventional culture media. Accordingly, ecology and biology Molecular Operational Taxonomic Units (MOTUs) Trypanosoma spp. Neobats unknown. Therefore, we performed molecular characterization targeting 18S small subunit rDNA from blood clot 280 bats three Brazilian regions (Paraíba, Rio de Janeiro Acre states), bypassing selective pressure hemoculture. From 68 (24%) positive samples, obtained 49 satisfactory sequences. Of these successfully sequenced results, T. (1, 3 4) represented 67%, most abundant sp. Neobat 4 (53%). Our results show: (1) high abundance wide geographic range 4, restricted Carollia bats; (2) infection rate perspicillata populations (mean 26%); (3) monoxenous Crithidia mellificae; (4) a new MOTU (T. 5) Artibeus cinereus, positioning wauwau clade. These data corroborate importance as hosts many C. mellificae. They also show that clade is underestimated warn about magnitude trypanosomes overpass findings combined previous include host-specific host-generalist species, probably playing different ecological roles: 1 shows broad host range; Carollia, respectively. Finally, seems be well-succeeded parasite, especially within metapopulations across geographical distribution. This work step forward understand life history Neobats.