Targeting the dynamic transcriptional landscape of Treg subpopulations in pancreatic ductal adenocarcinoma: Insights from single-cell RNA sequencing analysis with a focus on CTLA4 and TIGIT

• DOI: 10.1016/j.imbio.2024.152822
• Publication Date: 2024-06-05T08:35:05Z
• Authors: Adib Miraki Feriz; Arezou Khosrojerdi; Nafiseh Erfanian; Setareh Azarkar; Seyed Mehdi Sajjadi; Mohammad Javad Sh...; Mohammad Javad Va...; Hossein Safarpour; Vito Racanelli
• Keywords: TIGIT; Cancer Immunotherapy

Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy that represents significant challenge in cancer research and clinical management. In this study, we reanalyzed published single-cell RNA sequencing (scRNA-seq) dataset from PDAC adjacent tissues to investigate the heterogeneity of tumor normal tissue, specifically focusing on regulatory T cells (Tregs) their interactions with other microenvironment (TME). Treg were identified clustered into natural Tregs (nTreg) induced (iTreg) based expression specific genes. It was found number iTregs higher than healthy tissues, while n tissues. Differential gene analysis performed, biological process revealed mostly involved protein targeting translation pathways. addition, ligand-receptor pairs between cell types identified, critical communication pathways endothelial revealed, which could potentially contribute immunosuppressive TME PDAC. These findings provide insights role TME, highlighting potential targets for immunotherapy, such as inhibitory immune checkpoint receptors CTLA4 TIGIT, are known be expressed have been shown play suppressing anti-tumor responses.