M cell maturation and cDC activation determine the onset of adaptive immune priming in the neonatal Peyer’s patch

• DOI: 10.1016/j.immuni.2023.04.002
• Publication Date: 2023-05-01T14:47:25Z
• Authors: Natalia Torow; Ronghui Li; Thomas Charles Ad...; Clemens Mingels; Shahed Al Bounny; Niels van Best; Eva-Lena Stange; Britta Simons; Tiago Maié; Lennart Rüttger; Narasimha Murthy ...; Darryl Adelaide A...; Adam Benabid; Michael Gadermayr; Solveig Runge; Nicole Treichel; Dorit Merhof; Stephan Patrick R...; Nico Jehmlich; Timothy Wesley Hand; Martin von Bergen; Felix Heymann; Oliver Pabst; Thomas Clavel; Frank Tacke; Hugues Lelouard; Ivan Gesteira Costa; Mathias Walter Ho...
• Keywords: Peyer's patch; Priming (agriculture); Microfold cell

Early-life immune development is critical to long-term host health. However, the mechanisms that determine pace of postnatal maturation are not fully resolved. Here, we analyzed mononuclear phagocytes (MNPs) in small intestinal Peyer's patches (PPs), primary inductive site immunity. Conventional type 1 and 2 dendritic cells (cDC1 cDC2) RORgt+ antigen-presenting (RORgt+ APC) exhibited significant age-dependent changes subset composition, tissue distribution, reduced cell maturation, subsequently resulting a lack CD4+ T priming during period. Microbial cues contributed but could explain discrepancies MNP maturation. Type I interferon (IFN) accelerated IFN signaling did represent physiological stimulus. Instead, follicle-associated epithelium (FAE) M differentiation was required sufficient drive postweaning PP Together, our results highlight role FAE development.