Anti-allergic activity of R-phycocyanin from Porphyra haitanensis in antigen-sensitized mice and mast cells

• DOI: 10.1016/j.intimp.2015.02.032
• Publication Date: 2015-03-04T06:57:15Z
• Authors: Qingmei Liu; Youzhao Wang; Minjie Cao; Tzuming Pan; Yang Yang; Haiyan Mao; Lechang Sun; Guangming Liu
• Keywords: Porphyra; Mice, Inbred BALB C; 0303 health sciences; Phycocyanin; Tropomyosin; Immunoglobulin E; Cell Line; Rats; 3. Good health; Feces; 03 medical and health sciences; Jejunum; Th2 Cells; Anti-Allergic Agents; Hypersensitivity; Animals; Cytokines; Female; Mast Cells; Antigens; Cell Proliferation; Histamine

The prevalence of food allergy has increased in Asian countries. Marine algae have been proposed as the potential resource for anti-allergic therapeutics. The present study was aimed at isolating R-phycocyanin (RPC) from Porphyra haitanensis and determining the anti-allergy potential of RPC in antigen-sensitized mice and mast cells. In animal experiments, RPC could effectively reduce tropomyosin (TM)-specific immunoglobulin E (IgE) and histamine levels, alleviate allergy symptoms and jejunum tissue inflammation in mice, and inhibit the expression and release of cytokines (interleukin-4 (IL-4) and IL-13) in peritoneal lavage fluid. In spleen lymphocyte experiments, high purity of RPC skewed the immunological function of CD4(+) T cells towards Th1 activity. A higher expression of interferon (IFN)-γ was induced by a synergistic effect of TM and RPC. Through the Jun N-terminal kinase and Janus kinase 2 signaling pathways, IFN-γ synthesis was induced by RPC in combination with TM. Anti-allergic effect of RPC was evaluated in IgE-mediated rat mast RBL-2H3 cells. The results demonstrated that RPC inhibited allergy markers, including the release of β-hexosaminidase, histamine and ROS in antigen-sensitized RBL-2H3 cells. RPC also suppressed the production of pro-inflammatory factors (IL-4 and tumor necrosis factor-α). In conclusion, RPC decreased allergic sensitization against TM by blocking Th2 cell polarization as well as suppressed the release of allergic-mediators in antigen-stimulated mast cells. It may be used as a functional food component or active pharmaceutical ingredient for allergic patients.