3‑Bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol inhibits LPS-induced pro-inflammatory responses by preventing ROS production and downregulating NF-κB in vitro and in a zebrafish model

• DOI: 10.1016/j.intimp.2018.11.021
• Publication Date: 2018-12-08T11:30:24Z
• Authors: Eun-Yi Ko; Soo-Jin Heo; Su-Hyeon Cho; WonWoo Lee; Seo-Young Kim; Hye-Won Yang; Ginnae Ahn; Seon-Heui Cha; Seung-Hae Kwon; Myeong Seon Jeong; Kang Pa Lee; You-Jin Jeon; Kil-Nam Kim
• Keywords: Fish Proteins; Inflammation; Lipopolysaccharides; 0301 basic medicine; Embryo, Nonmammalian; Anti-Inflammatory Agents; NF-kappa B; Down-Regulation; Nitric Oxide Synthase Type II; Benzene; Nitric Oxide; Disease Models, Animal; Mice; 03 medical and health sciences; RAW 264.7 Cells; Rhodophyta; Animals; Humans; Reactive Oxygen Species; Zebrafish

The anti-inflammatory effects of 3‑bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol (BEMB) from Polysiphonia morrowii were evaluated in lipopolysaccharide (LPS)-induced RAW264.7 cells and zebrafish embryo. BEMB showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO) and reactive oxygen species (ROS), and the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in the LPS-activated RAW264.7 cells and zebrafish embryo without cytotoxicity. Moreover, BEMB suppressed the protein and mRNA expression levels of nuclear factor (NF)-κB (p65 and inhibitor of NF-κB [IκB]-A) in RAW264.7 cells and zebrafish embryo, respectively. Collectively, the results of this study indicate that BEMB suppressed the production of pro-inflammatory mediators such as NO, iNOS, and COX-2 as well as their regulation in LPS-induced RAW264.7 cells and zebrafish embryos by inhibiting ROS production and NF-κB expression. Therefore, this study suggests that BEMB could be a potential anti-inflammatory agent for the treatment of inflammatory diseases.