Elucidating the molecular pathways and immune system transcriptome during ischemia-reperfusion injury in renal transplantation

• DOI: 10.1016/j.intimp.2020.106246
• Publication Date: 2020-02-07T11:59:30Z
• Authors: Jinhua Zhang; Xiangling Wei; Zuofu Tang; Bin Miao; Yingxun Luo; Xiao Hu; You Luo; Yu Zhou; Ning Na
• Keywords: KEGG; Biological pathway

Ischemia reperfusion injury (IRI) is a major challenge for renal transplantation. This study was performed to explore the mechanisms and potential molecular targets involved in IRI. In this study, gene datasets GSE43974 GSE126805 from Gene Expression Omnibus database, which include ischemic reperfused specimens, were analyzed determine differentially expressed genes (DEGs). ontology annotations, Kyoto Encyclopedia of Genes Genomes analysis, set enrichment analysis pathways that are significantly enriched during ischemia reperfusion. We also determined microenvironment cell types xCell correlation analyses reveal relationship between infiltration. found 77 DEGs (76 up- 1 downregulated) 323 (312 11 datasets, respectively. Similar signaling pathway patterns observed two datasets. The combined demonstrate NOD-like receptor its downstream pathways, MAPK NF-kβ, pathways. identified immune cells changed after reperfusion, including hematopoietic stem cells, M2 macrophages, monocytes, Treg conventional dendritic pro B-cells. Enrichment scores IRI correlated with change levels class-switched memory B-cell both These data important role IRI, close infiltration specific types. Our provide compelling insights into pathogenesis therapeutic